MycorrhizaeV1, Main, Exploration, bibRecord, 000F54

The Thiamine Biosynthesis Gene THI1 Promotes Nodule Growth and Seed Maturation.

Identifieur interne : 000F54 ( Main/Exploration ); précédent : 000F53; suivant : 000F55

The Thiamine Biosynthesis Gene THI1 Promotes Nodule Growth and Seed Maturation.

Auteurs : Miwa Nagae [Allemagne] ; Martin Parniske [Allemagne] ; Masayoshi Kawaguchi [Allemagne] ; Naoya Takeda [Japon]

Source :

Plant physiology [ 1532-2548 ] ; 2016.

RBID : pubmed:27702844

Descripteurs français

KwdFr :
- Feuilles de plante (effets des médicaments et des substances chimiques), Feuilles de plante (métabolisme), Fractions subcellulaires (métabolisme), Graines (croissance et développement), Graines (effets des médicaments et des substances chimiques), Graines (génétique), Gènes de plante (MeSH), Loteae (génétique), Loteae (microbiologie), Mutation (génétique), Mycorhizes (effets des médicaments et des substances chimiques), Mycorhizes (métabolisme), Nodules racinaires de plante (croissance et développement), Nodules racinaires de plante (effets des médicaments et des substances chimiques), Nodules racinaires de plante (métabolisme), Numération de colonies microbiennes (MeSH), Phénotype (MeSH), Plastes (métabolisme), Pousses de plante (anatomie et histologie), Pousses de plante (effets des médicaments et des substances chimiques), Protéines végétales (génétique), Protéines végétales (métabolisme), Rhizobium (croissance et développement), Rhizobium (effets des médicaments et des substances chimiques), Régulation de l'expression des gènes végétaux (effets des médicaments et des substances chimiques), Symbiose (MeSH), Techniques de knock-down de gènes (MeSH), Thiamine (biosynthèse), Thiamine (pharmacologie), Voies de biosynthèse (génétique).
MESH :
- anatomie et histologie : Pousses de plante.
- biosynthèse : Thiamine.
- croissance et développement : Graines, Nodules racinaires de plante, Rhizobium.
- effets des médicaments et des substances chimiques : Feuilles de plante, Graines, Mycorhizes, Nodules racinaires de plante, Pousses de plante, Rhizobium, Régulation de l'expression des gènes végétaux.
- génétique : Graines, Loteae, Mutation, Protéines végétales, Voies de biosynthèse.
- microbiologie : Loteae.
- métabolisme : Feuilles de plante, Fractions subcellulaires, Mycorhizes, Nodules racinaires de plante, Plastes, Protéines végétales.
- pharmacologie : Thiamine.
- Gènes de plante, Numération de colonies microbiennes, Phénotype, Symbiose, Techniques de knock-down de gènes.

English descriptors

KwdEn :
- Biosynthetic Pathways (genetics), Colony Count, Microbial (MeSH), Gene Expression Regulation, Plant (drug effects), Gene Knockdown Techniques (MeSH), Genes, Plant (MeSH), Lotus (genetics), Lotus (microbiology), Mutation (genetics), Mycorrhizae (drug effects), Mycorrhizae (metabolism), Phenotype (MeSH), Plant Leaves (drug effects), Plant Leaves (metabolism), Plant Proteins (genetics), Plant Proteins (metabolism), Plant Shoots (anatomy & histology), Plant Shoots (drug effects), Plastids (metabolism), Rhizobium (drug effects), Rhizobium (growth & development), Root Nodules, Plant (drug effects), Root Nodules, Plant (growth & development), Root Nodules, Plant (metabolism), Seeds (drug effects), Seeds (genetics), Seeds (growth & development), Subcellular Fractions (metabolism), Symbiosis (MeSH), Thiamine (biosynthesis), Thiamine (pharmacology).
MESH :
- chemical , biosynthesis : Thiamine.
- chemical , genetics : Plant Proteins.
- anatomy & histology : Plant Shoots.
- drug effects : Gene Expression Regulation, Plant, Mycorrhizae, Plant Leaves, Plant Shoots, Rhizobium, Root Nodules, Plant, Seeds.
- genetics : Biosynthetic Pathways, Lotus, Mutation, Seeds.
- growth & development : Rhizobium, Root Nodules, Plant, Seeds.
- metabolism : Mycorrhizae, Plant Leaves, Plant Proteins, Plastids, Root Nodules, Plant, Subcellular Fractions.
- microbiology : Lotus.
- chemical , pharmacology : Thiamine.
- Colony Count, Microbial, Gene Knockdown Techniques, Genes, Plant, Phenotype, Symbiosis.

Abstract

Thiamine (vitamin B1) is essential for living organisms. Unlike animals, plants can synthesize thiamine. In Lotus japonicus, the expression of two thiamine biosynthesis genes, THI1 and THIC, was enhanced by inoculation with rhizobia but not by inoculation with arbuscular mycorrhizal fungi. THIC and THI2 (a THI1 paralog) were expressed in uninoculated leaves. THI2-knockdown plants and the transposon insertion mutant thiC had chlorotic leaves. This typical phenotype of thiamine deficiency was rescued by an exogenous supply of thiamine. In wild-type plants, THI1 was expressed mainly in roots and nodules, and the thi1 mutant had green leaves even in the absence of exogenous thiamine. THI1 was highly expressed in actively dividing cells of nodule primordia. The thi1 mutant had small nodules, and this phenotype was rescued by exogenous thiamine and by THI1 complementation. Exogenous thiamine increased nodule diameter, but the level of arbuscular mycorrhizal colonization was unaffected in the thi1 mutant or by exogenous thiamine. Expression of symbiotic marker genes was induced normally, implying that mainly nodule growth was delayed in the thi1 mutant. Furthermore, this mutant formed many immature seeds with reduced seed weight. These results indicate that thiamine biosynthesis mediated by THI1 enhances nodule enlargement and is required for seed development in L. japonicus.

DOI: 10.1104/pp.16.01254
PubMed: 27702844
PubMed Central: PMC5100774

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 000F09
to stream Main, to step Curation: 000F09

Le document en format XML

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<series><title level="j">Plant physiology</title>
<idno type="eISSN">1532-2548</idno>
<imprint><date when="2016" type="published">2016</date>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biosynthetic Pathways (genetics)</term>
<term>Colony Count, Microbial (MeSH)</term>
<term>Gene Expression Regulation, Plant (drug effects)</term>
<term>Gene Knockdown Techniques (MeSH)</term>
<term>Genes, Plant (MeSH)</term>
<term>Lotus (genetics)</term>
<term>Lotus (microbiology)</term>
<term>Mutation (genetics)</term>
<term>Mycorrhizae (drug effects)</term>
<term>Mycorrhizae (metabolism)</term>
<term>Phenotype (MeSH)</term>
<term>Plant Leaves (drug effects)</term>
<term>Plant Leaves (metabolism)</term>
<term>Plant Proteins (genetics)</term>
<term>Plant Proteins (metabolism)</term>
<term>Plant Shoots (anatomy & histology)</term>
<term>Plant Shoots (drug effects)</term>
<term>Plastids (metabolism)</term>
<term>Rhizobium (drug effects)</term>
<term>Rhizobium (growth & development)</term>
<term>Root Nodules, Plant (drug effects)</term>
<term>Root Nodules, Plant (growth & development)</term>
<term>Root Nodules, Plant (metabolism)</term>
<term>Seeds (drug effects)</term>
<term>Seeds (genetics)</term>
<term>Seeds (growth & development)</term>
<term>Subcellular Fractions (metabolism)</term>
<term>Symbiosis (MeSH)</term>
<term>Thiamine (biosynthesis)</term>
<term>Thiamine (pharmacology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Feuilles de plante (effets des médicaments et des substances chimiques)</term>
<term>Feuilles de plante (métabolisme)</term>
<term>Fractions subcellulaires (métabolisme)</term>
<term>Graines (croissance et développement)</term>
<term>Graines (effets des médicaments et des substances chimiques)</term>
<term>Graines (génétique)</term>
<term>Gènes de plante (MeSH)</term>
<term>Loteae (génétique)</term>
<term>Loteae (microbiologie)</term>
<term>Mutation (génétique)</term>
<term>Mycorhizes (effets des médicaments et des substances chimiques)</term>
<term>Mycorhizes (métabolisme)</term>
<term>Nodules racinaires de plante (croissance et développement)</term>
<term>Nodules racinaires de plante (effets des médicaments et des substances chimiques)</term>
<term>Nodules racinaires de plante (métabolisme)</term>
<term>Numération de colonies microbiennes (MeSH)</term>
<term>Phénotype (MeSH)</term>
<term>Plastes (métabolisme)</term>
<term>Pousses de plante (anatomie et histologie)</term>
<term>Pousses de plante (effets des médicaments et des substances chimiques)</term>
<term>Protéines végétales (génétique)</term>
<term>Protéines végétales (métabolisme)</term>
<term>Rhizobium (croissance et développement)</term>
<term>Rhizobium (effets des médicaments et des substances chimiques)</term>
<term>Régulation de l'expression des gènes végétaux (effets des médicaments et des substances chimiques)</term>
<term>Symbiose (MeSH)</term>
<term>Techniques de knock-down de gènes (MeSH)</term>
<term>Thiamine (biosynthèse)</term>
<term>Thiamine (pharmacologie)</term>
<term>Voies de biosynthèse (génétique)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Thiamine</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="anatomie et histologie" xml:lang="fr"><term>Pousses de plante</term>
</keywords>
<keywords scheme="MESH" qualifier="anatomy & histology" xml:lang="en"><term>Plant Shoots</term>
</keywords>
<keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Thiamine</term>
</keywords>
<keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Graines</term>
<term>Nodules racinaires de plante</term>
<term>Rhizobium</term>
</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Gene Expression Regulation, Plant</term>
<term>Mycorrhizae</term>
<term>Plant Leaves</term>
<term>Plant Shoots</term>
<term>Rhizobium</term>
<term>Root Nodules, Plant</term>
<term>Seeds</term>
</keywords>
<keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Feuilles de plante</term>
<term>Graines</term>
<term>Mycorhizes</term>
<term>Nodules racinaires de plante</term>
<term>Pousses de plante</term>
<term>Rhizobium</term>
<term>Régulation de l'expression des gènes végétaux</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Biosynthetic Pathways</term>
<term>Lotus</term>
<term>Mutation</term>
<term>Seeds</term>
</keywords>
<keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Rhizobium</term>
<term>Root Nodules, Plant</term>
<term>Seeds</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Graines</term>
<term>Loteae</term>
<term>Mutation</term>
<term>Protéines végétales</term>
<term>Voies de biosynthèse</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Mycorrhizae</term>
<term>Plant Leaves</term>
<term>Plant Proteins</term>
<term>Plastids</term>
<term>Root Nodules, Plant</term>
<term>Subcellular Fractions</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Loteae</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Lotus</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Feuilles de plante</term>
<term>Fractions subcellulaires</term>
<term>Mycorhizes</term>
<term>Nodules racinaires de plante</term>
<term>Plastes</term>
<term>Protéines végétales</term>
</keywords>
<keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Thiamine</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Thiamine</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Colony Count, Microbial</term>
<term>Gene Knockdown Techniques</term>
<term>Genes, Plant</term>
<term>Phenotype</term>
<term>Symbiosis</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Gènes de plante</term>
<term>Numération de colonies microbiennes</term>
<term>Phénotype</term>
<term>Symbiose</term>
<term>Techniques de knock-down de gènes</term>
</keywords>
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<front><div type="abstract" xml:lang="en">Thiamine (vitamin B1) is essential for living organisms. Unlike animals, plants can synthesize thiamine. In Lotus japonicus, the expression of two thiamine biosynthesis genes, THI1 and THIC, was enhanced by inoculation with rhizobia but not by inoculation with arbuscular mycorrhizal fungi. THIC and THI2 (a THI1 paralog) were expressed in uninoculated leaves. THI2-knockdown plants and the transposon insertion mutant thiC had chlorotic leaves. This typical phenotype of thiamine deficiency was rescued by an exogenous supply of thiamine. In wild-type plants, THI1 was expressed mainly in roots and nodules, and the thi1 mutant had green leaves even in the absence of exogenous thiamine. THI1 was highly expressed in actively dividing cells of nodule primordia. The thi1 mutant had small nodules, and this phenotype was rescued by exogenous thiamine and by THI1 complementation. Exogenous thiamine increased nodule diameter, but the level of arbuscular mycorrhizal colonization was unaffected in the thi1 mutant or by exogenous thiamine. Expression of symbiotic marker genes was induced normally, implying that mainly nodule growth was delayed in the thi1 mutant. Furthermore, this mutant formed many immature seeds with reduced seed weight. These results indicate that thiamine biosynthesis mediated by THI1 enhances nodule enlargement and is required for seed development in L. japonicus.</div>
</front>
</TEI>
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<ISOAbbreviation>Plant Physiol</ISOAbbreviation>
</Journal>
<ArticleTitle>The Thiamine Biosynthesis Gene THI1 Promotes Nodule Growth and Seed Maturation.</ArticleTitle>
<Pagination><MedlinePgn>2033-2043</MedlinePgn>
</Pagination>
<Abstract><AbstractText>Thiamine (vitamin B1) is essential for living organisms. Unlike animals, plants can synthesize thiamine. In Lotus japonicus, the expression of two thiamine biosynthesis genes, THI1 and THIC, was enhanced by inoculation with rhizobia but not by inoculation with arbuscular mycorrhizal fungi. THIC and THI2 (a THI1 paralog) were expressed in uninoculated leaves. THI2-knockdown plants and the transposon insertion mutant thiC had chlorotic leaves. This typical phenotype of thiamine deficiency was rescued by an exogenous supply of thiamine. In wild-type plants, THI1 was expressed mainly in roots and nodules, and the thi1 mutant had green leaves even in the absence of exogenous thiamine. THI1 was highly expressed in actively dividing cells of nodule primordia. The thi1 mutant had small nodules, and this phenotype was rescued by exogenous thiamine and by THI1 complementation. Exogenous thiamine increased nodule diameter, but the level of arbuscular mycorrhizal colonization was unaffected in the thi1 mutant or by exogenous thiamine. Expression of symbiotic marker genes was induced normally, implying that mainly nodule growth was delayed in the thi1 mutant. Furthermore, this mutant formed many immature seeds with reduced seed weight. These results indicate that thiamine biosynthesis mediated by THI1 enhances nodule enlargement and is required for seed development in L. japonicus.</AbstractText>
<CopyrightInformation>© 2016 American Society of Plant Biologists. All Rights Reserved.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Nagae</LastName>
<ForeName>Miwa</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>Division of Symbiotic Systems, National Institute for Basic Biology, Myodaiji, Okazaki, Aichi 444-8585, Japan (M.N., M.K., N.T.).</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Genetics, Faculty of Biology, University of Munich, 82152 Martinsried, Germany (M.P.); and.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Department of Basic Biology, School of Life Science, Graduate University for Advanced Studies, Myodaiji, Okazaki, Aichi 444-8585, Japan (M.K., N.T.).</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Parniske</LastName>
<ForeName>Martin</ForeName>
<Initials>M</Initials>
<Identifier Source="ORCID">0000-0001-8561-747X</Identifier>
<AffiliationInfo><Affiliation>Division of Symbiotic Systems, National Institute for Basic Biology, Myodaiji, Okazaki, Aichi 444-8585, Japan (M.N., M.K., N.T.).</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Genetics, Faculty of Biology, University of Munich, 82152 Martinsried, Germany (M.P.); and.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Department of Basic Biology, School of Life Science, Graduate University for Advanced Studies, Myodaiji, Okazaki, Aichi 444-8585, Japan (M.K., N.T.).</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kawaguchi</LastName>
<ForeName>Masayoshi</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>Division of Symbiotic Systems, National Institute for Basic Biology, Myodaiji, Okazaki, Aichi 444-8585, Japan (M.N., M.K., N.T.).</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Genetics, Faculty of Biology, University of Munich, 82152 Martinsried, Germany (M.P.); and.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Department of Basic Biology, School of Life Science, Graduate University for Advanced Studies, Myodaiji, Okazaki, Aichi 444-8585, Japan (M.K., N.T.).</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Takeda</LastName>
<ForeName>Naoya</ForeName>
<Initials>N</Initials>
<AffiliationInfo><Affiliation>Division of Symbiotic Systems, National Institute for Basic Biology, Myodaiji, Okazaki, Aichi 444-8585, Japan (M.N., M.K., N.T.); takedan@nibb.ac.jp.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Genetics, Faculty of Biology, University of Munich, 82152 Martinsried, Germany (M.P.); and takedan@nibb.ac.jp.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Department of Basic Biology, School of Life Science, Graduate University for Advanced Studies, Myodaiji, Okazaki, Aichi 444-8585, Japan (M.K., N.T.) takedan@nibb.ac.jp.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2016</Year>
<Month>10</Month>
<Day>04</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>United States</Country>
<MedlineTA>Plant Physiol</MedlineTA>
<NlmUniqueID>0401224</NlmUniqueID>
<ISSNLinking>0032-0889</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>X66NSO3N35</RegistryNumber>
<NameOfSubstance UI="D013831">Thiamine</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D053898" MajorTopicYN="N">Biosynthetic Pathways</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D015169" MajorTopicYN="N">Colony Count, Microbial</DescriptorName>
</MeshHeading>
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<QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName>
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<MeshHeading><DescriptorName UI="D017343" MajorTopicYN="N">Genes, Plant</DescriptorName>
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<MeshHeading><DescriptorName UI="D010641" MajorTopicYN="N">Phenotype</DescriptorName>
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<MeshHeading><DescriptorName UI="D012231" MajorTopicYN="N">Rhizobium</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName>
<QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName>
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<MeshHeading><DescriptorName UI="D053204" MajorTopicYN="N">Root Nodules, Plant</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName>
<QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
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<MeshHeading><DescriptorName UI="D012639" MajorTopicYN="N">Seeds</DescriptorName>
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<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
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<MeshHeading><DescriptorName UI="D013559" MajorTopicYN="N">Symbiosis</DescriptorName>
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<Month>08</Month>
<Day>12</Day>
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The Thiamine Biosynthesis Gene THI1 Promotes Nodule Growth and Seed Maturation.

The Thiamine Biosynthesis Gene THI1 Promotes Nodule Growth and Seed Maturation.

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